wikiHow to Calibrate and Use a pH Meter

Chemists, biologists, environmentalists and lab technicians alike all use pH to measure the acidic and basic potentials of a solution. A pH meter is very useful and the most accurate tool available to test pH levels. Here’s how to calibrate and use a basic pH meter in an easy step-by-step method. dd


For calibrating the PH of water using other methods, check out "How to Measure the pH of Water."

Steps

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    Turn on pH meter.
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    Wait around 30 minutes for the electronics to warm up.
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    Take electrode out of storage solution.
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    Rinse the electrode with distilled water. Do not wipe the electrode membrane, but instead dab the electrode with Kimwipe®.
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    Submerse the electrode into the pH 7 buffer. Note that buffers and samples should be read at room temperature.
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    Press the calibrate button.
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    Wait until the pH icon stops flashing and press the calibrate button again.
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    Rinse the electrode with distilled water and wipe with Kimwipe®.
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    Submerse the electrode into the pH 4 buffer. It is not recommended to use buffer 10 due to poor stability as a result buffer reaction with CO2 from atmosphere.
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    Wait until the pH icon stops flashing and press the measure button.
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    Rinse the electrode with distilled water and dab dry with Kimwipe®.
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    Submerse the electrode into your sample. Note that the sample and buffers should be read at room temperature.
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    Press the measure button.
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    Wait until the pH icon stops flashing and record the pH of your sample.

Tips

  • Rinse the electrode with distilled water and dab dry with Kimwipe®, after taking each measurement, to prevent contaminants in the samples.
  • Don't touch the glass membrane(at the end )with your finger because the glass membrane is too sensitive.
  • Press the ‘Measure’ knob after you submerse each sample.

Warnings

  • Follow all other set safety regulations when working with hazardous samples.
  • Wear gloves when testing pH.

Things You'll Need

  • pH meter
  • Distilled water
  • Kimwipe®, a special thin tissue specifically for laboratory use
  • pH 7 buffer, a solution whose pH is constantly 7
  • pH 4 buffer, a solution whose pH is constantly 4

Article Info

Categories: Aquariums | Chemistry